OJFR  >> Vol. 3 No. 4 (December 2016)

    烟酸诺氟沙星对乌鳢肝微粒酶活性的影响
    The Influence of the Different Doses of Niacin Norfloxacin on the Enzyme Activity of Hepatic Microsome in Channa argus

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作者:  

左梦丽,沈锦玉:浙江省海水水产研究所,浙江省鱼类安康与养分重点实验室,浙江 湖州;上海陆地大年夜学水产与生命学院,上海;
姚嘉赟:浙江省海水水产研究所,浙江省鱼类安康与养分重点实验室,浙江 湖州;
尹文林:上海陆地大年夜学水产与生命学院,上海

关键词:
乌鳢烟酸诺氟沙星肝微粒体酶活性Channa argus Niacin Norfloxacin Hepatic Microsome Enzyme Activity

摘要:

目标:研究不合浓度烟酸诺氟沙星对乌鳢肝微粒体细胞色素P450酶系的影响,旨在为水产养殖公道用药供给安然指导。办法:安康乌鳢60尾,体重200 ± 20.0 g,随机分为空白对比组、低剂量(5 mg/kg)、中剂量(20 mg/kg)、高剂量(40 mg/kg)烟酸诺氟沙星浓度组,每组15尾,持续口灌给药5天。采取差速离心法提取肝微粒体,应用底物代谢法结合紫外分光光度计测定NADPH-细胞色素C复原酶(NCCR)、苯胺-4-羟化酶(AH)、氨基比林-N-去甲基酶(AND)、红霉素-N-脱甲基酶(ERND) 4种酶的含量。成果实验成果注解,与对比组比拟,烟酸诺氟沙星组微粒体蛋白的含量明显增添(p < 0.05),而NCCR、AH、AND、ERND4种酶的活性则明显进步(p < 0.05)。且随着药物浓度的降低,表示出活性加强的趋势。结论:烟酸诺氟沙星对乌鳢肝微粒体细胞色素P450酶系活性具有引诱感化。

The present study was conducted to evaluate the effects of niacin norfloxacin on the activity of CYP450 enzyme in liver of Channa argus. The aim of the study was to provide security guidance for rational use of niacin norfloxacin in aquaculture. C. arfus were fed with different concentrations (0 mg/ml, 5 mg/kg, 20 mg/kg, 40 mg/kg) of niacin norfloxacin by oral administration for 5 days. The activities of enzyme of NCCR, AH, AND and ERND were determined. Our results demonstrated that microsomal protein content in niacin norfloxacin groups were decreased significantly as compared with control group, and the activities of NCCR, AH, AND and ERND were significantly increased in a dose-depended model which indicated that niacin norfloxacin could promote the activity of those enzyme.

文章援用:
左梦丽, 姚嘉赟, 沈锦玉, 尹文林. 烟酸诺氟沙星对乌鳢肝微粒酶活性的影响[J]. 水产研究, 2016, 3(4): 88-96. http://dx.doi.org/10.12677/OJFR.2016.34014

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